悪性黒色腫の代謝活性による原子炉治療の展開に関する研究
Selective Thermal Neutron Capture Treatment of Malignant Melanoma Using its Specific Metabolic Activity

1985年1月　(January, 1985)
編集：三島豊
Edited by： Yutaka Mishima

目次

まえがき /  研究代表者 神戸大学医学部 教授 三島豊  /p.Ⅺ

1. Cure of Malignant Melanoma by Single Thermal Neutron Capture Treatment Using Melanoma-Seeking Compounds : ¹⁰B/Melanogenesis Interaction to In Vitro/In Vivo Radiobiological Analysis to Preclinical Studies / Yutaka Mishima, Masamitsu Ichihashi, Takafumi Nakanishi, Masayuki Tsuji, Masato Ueda, Susumu Hatta, Toshio Nakagawa (Department of Dermatology, Kobe University School of Medicine), Tatsuo Suzuki, Tsunenori Suganuma and Kohsaku Kobayashi (School of Veterinary Medicine, Azabu University)  /p.1
2. 熱中性子捕捉療法における黒色腫担癌ハムスクーの転移解析 /  上田正登， 三島豊， 市橋正光（ 神戸大医）  /p.15
3. In Vitro Radio biological Evaluation of Selective Killing Effects of ¹⁰B₁ -para-boronophenylalanine・HCl in the Thermal Neutron Capture Therapy of Malignant Melanoma Cells / Masamitsu Ichihashi, Masato Ueda, Kazuhito Hayashibe, Susumu Hatta, Masayuki Tsuji, Yu taka Mishima (Department of Dermatology, Kobe University School of Medicine), Hiroshi Fukuda (Department of Radiology and Nuclear Medicine, The Research Institute for Tuberculosis and Cancer, Tohoku University), Tooru Kobayashi and Keiji Kanda (Research Reactor Institute, Kyoto University)  /p.23
4. 4Specific Killing Effect of ¹⁰B₁Para-boronophenylalaninein Thermal Neutron Capture Therapy of Malignant Melanoma : In Vitro Radiobiological Evaluation / Masamitsu Ichihashi, Takafumi Nakanishi and Yutaka Mishima (Department of Dermatology, Kobe University School of Medicine)  /p.33
5. ₁₀B₁ -paraboronophenylalanine・HClの選択的黒色腫親和性：熱中性子捕捉療法のために / 辻正幸、市橋正光、三島豊（神戸大医）  /p.37
6. Thermal Neutron Capture Therapy of Malignant Melanoma : in vitro Radiobiological Analysis / Takafumi Nakanishi, Masamitsu Ichihashi, Yutaka Mishima (Department of Dermatology, Kobe University School of Medicine), Taiju Matsuzawa and Hiroshi Fukuda (Department of Radiology and Nuclear Medicine, The Research Institute for Tuberculosis and Cancer, Tohoku University)  /p.43
7. ₁₀B₁-paraboronophenylalanineの生体内動態アミノ酸自動分析及び薄層クロマトグラフィー法 / 藤原寛， 藤井芳夫， 加藤紀子， 辻正幸, 市橋正光、三島豊、田中千賀子（神戸大医）  /p.51
8. ₁₀B₁-paraboronophenylalanineの微量定量 / 藤原寛、藤井芳夫、田中千賀子、市橋正光、三島豊（神戸大医）  /p.55
9. 黒色腫担癌ハムスクーの選択的熱中性子捕捉療法ー電子線照射との比較解析 / 平塚純一、木村修治（神戸大医）辛嶋博（兵庫成人病センター）三島豊（神戸大医）  /p.59
10. 原子炉による熱中性子捕捉療法の臨床に向けての人骨入り人体Phantom照射実験に於ける線量測定 / 辛嶋博（兵庫成人病センター）平塚純一、三島豊（神戸大医）  /p.63
11. α-トラック法によるBoron-10の体内分布測定 / 高垣政雄（山形大医）、三島豊， 市橋正光（神戸大医）福田寛、松沢大樹（東北大）  /p.69
12. Boronic, Diboronic and a-Amino.-boronic Acids Derived from l-Ethynyl-3, 4 dimethoxybenzene / Toshia Nakagawa and Yu taka Mishima (Department of Dermatology, Kobe University School of Medicine)  /p.73
13. 悪性黒色腫の速中性子線治療臨床トライアル / 恒元博、森田新六、石川達雄（放射線医研）  /p.83
14. ヌードマウス可移植ヒト黒色腫株による選択的熱中性子捕捉治療モデルの検討 / 玉内秀一、玉置憲一（東海大医）上田正登、三島豊（神戸大医）  /p.91
15. Shared Antigenic Determinant Expressed on Various Mammalian Melanoma Cells / Masaru Taniguchi (Department of Immunology, Chiba University School of Medicine) and Seiji Wakabayashi (Department of Dermatology, Chiba University School of Medicine)  /p.95
16. Sandwich Radioimmunoassay Using Single Monoclonal Antibody That Detects Minute Amounts of Melanoma Antigens from Various Mammalian Species / Seiji Wakabayashi, Shoji Okamoto (Department of Dermatology, Chiba University School of Medicine) and Masaru Taniguchi (Department of Immunology, Chiba University School of Medicine)  /p.105
17. 生体ファントム内の熱中性子分布 / 柴田俊一、佐藤孝司、小野光ー（京大原子炉）  /p.111
18. 中性子捕獲療法における物理工学面の進歩 生体中のppmオーダ¹⁰B濃度の測定法の開発と重水の(γ, n)反応の中性子場に与える影響の 評価 / 神田啓治、古林徹、青木一彦（京大原子炉）  /p.117
19. 中性子捕獲療法における選択的照射技術および照射線量評価の原子炉物理工学的検討/ 古林徹、神田啓治（京大原子炉）  /p.133
20. Analytical Calculation of Boron-10 Dosage in Cell Nucleus for Neutron Capture Therapy /Tooru Kobayashi and Keiji Kanda (Research Reactor Institute, Kyoto University)  /p.145
21. Microanalysis System of ppm-order ¹⁰B Concentrations in Tissue for Neutron Capture Therapy by Prompt Gamma-ray Spectrometry / Tooru Kobayashi and Keiji Kanda (Research Reactor Institute, Kyoto University)  /p.163
22. Phantom Experiment and Analysis for in vivo Measurement of Boron-10 Concentrations in Melanoma for Boron Neutron Capture Therapy / Kazuhiko Aoki, Tooru Kobayashi, and Keiji Kanda (Research Reactor Institute, Kyoto University)  /p.171
23. 黒色腫の選択的熱中性子捕捉療法における細胞致死効果の生物学的研究 / 江口吾朗、児玉隆治、阿形清和、黒岩常祥（岡崎基礎生研）岡本光正（名大理）市橋正光、三島豊（神戸大医）  /p.181
24. Regeneration of the Lens as a Phenomenon of Cellular Transdifferentiation : Regulability of the Differentiated State of the Vertebrate Pigment Epithelial Cell / Goro Eguchi (Institute of Molecular Biology, Faculty of Science, Nagoya University) and Yoshiaki ltoh (Laboratory for Biology, Aichi Medical University)  /p.191
25. 熱中性子捕捉反応のRBEの評価について一数式計算モデルによる評価－ / 福田寛（東北大）古林徹、神田啓治（京大原子炉）松澤大樹（東北大）  /p.197
26. 培養細胞の熱中性子照射実験における中性子フルエンスの測定について / 福田寛（東北大）古林徹（京大原子炉）市橋正光、三島豊（神戸大医）松澤大樹（東北大）  /p.203
27. 熱中性子捕捉療法における障害修復機構の解析/ 内海博司（京大医）市橋正光、三島豊（神戸大医）  /p.209
28. Enhancement of Thermal Neutron-Induced Killing Effect on HeLa Cells by Means of Three 10-Boron-Compounds / Yowri Jeno (Department of Experimental Radiology, Faculty of Medicine, Kyoto University)  /p.219
29. ¹¹B, ¹³C-NMR Study of the Complex Formation of Phenylboronate with Catechol and L-Dopa / Kazuo Yoshino, Satoshi Oguchi, Yuji Shimakata, Yoshihiro Mori (Department of Chemistry, Shinshu University), Makoto Okamoto, Masahiro Kotaka (Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology) and Hidetake Kakihana (Department of Chemistry, Sophia University)  /p.221
30. 生体組織中の微量ホウ素の化学分析による，Melanoma-seeking agent, ¹⁰B₁-p-Borono- phenylalanine・HCIの研究 /吉野和夫（ 信州大理）岡本真実（東工大原子炉）垣花秀武（上智大理）森芳弘（信州大理）三島豊、市橋正光、辻正幸、中西孝文（神戸大医）  /p.233
31. Spectrophotometric Determination of Trace Boron in Biological Materials after Alkali Fusion Decomposition /Kazuo Yoshino, Makoto Okamoto, and Hidetake Kakihana (Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology) Takafumi Nakanishi, Masamitsu Ichihashi, and Yutaka Mishima (Department of Dermatology, Kobe University School of Medicine)  /p.247
32. ¹⁰B₁ -paraboronophenylalanine・HClのマウスにおける急性毒性 / 谷山紘太郎、藤原寛、田中千賀子（神戸大医）  /p.251

Conents

Preface /  Preface Yut aka Mishima (Department of Dermatology, Kobe University School of Medicine  /p.Ⅺ

1. Cure of Malignant Melanoma by Single Thermal Neutron Capture Treatment Using Melanoma-Seeking Compounds : ¹⁰B/Melanogenesis Interaction to In Vitro/In Vivo Radiobiological Analysis to Preclinical Studies / Yutaka Mishima, Masamitsu Ichihashi, Takafumi Nakanishi, Masayuki Tsuji, Masato Ueda, Susumu Hatta, Toshio Nakagawa (Department of Dermatology, Kobe University School of Medicine), Tatsuo Suzuki, Tsunenori Suganuma and Kohsaku Kobayashi (School of Veterinary Medicine, Azabu University)  /p.1
2. Effects of Thermal Neutron Capture Therapy on Melanoma Metastasis /  Masato Ueda, Yutaka Mishima and Masamitsu Ichihashi (Department of Dermatology, Kobe University School of Medicine)  /p.15
3. In Vitro Radio biological Evaluation of Selective Killing Effects of ¹⁰B₁ -para-boronophenylalanine・HCl in the Thermal Neutron Capture Therapy of Malignant Melanoma Cells / Masamitsu Ichihashi, Masato Ueda, Kazuhito Hayashibe, Susumu Hatta, Masayuki Tsuji, Yu taka Mishima (Department of Dermatology, Kobe University School of Medicine), Hiroshi Fukuda (Department of Radiology and Nuclear Medicine, The Research Institute for Tuberculosis and Cancer, Tohoku University), Tooru Kobayashi and Keiji Kanda (Research Reactor Institute, Kyoto University)  /p.23
4. Specific Killing Effect of ¹⁰B₁Para-boronophenylalaninein Thermal Neutron Capture Therapy of Malignant Melanoma : In Vitro Radiobiological Evaluation / Masamitsu Ichihashi, Takafumi Nakanishi and Yutaka Mishima (Department of Dermatology, Kobe University School of Medicine)  /p.33
5. Selective Affinity of ¹⁰B₁– paraboronophenylalanine・HCIto Malignant Melanoma; In Relation to Thermal Neutron Capture Therapy / Masayuki Tsuji, Masamitsu Ichihashi and Yutaka Mishima (Department of Dermatology, Kobe University School of Medicine)  /p.37
6. Thermal Neutron Capture Therapy of Malignant Melanoma : in vitro Radiobiological Analysis / Takafumi Nakanishi, Masamitsu Ichihashi, Yutaka Mishima (Department of Dermatology, Kobe University School of Medicine), Taiju Matsuzawa and Hiroshi Fukuda (Department of Radiology and Nuclear Medicine, The Research Institute for Tuberculosis and Cancer, Tohoku University)  /p.43
7. Dynamics of¹⁰B₁ paraboronophenylalanine Accumulation, Metabolism and Excretion : Amino Acid Auto analytical and Thin Layer Chromatographic Studies / Hiroshi Fujiwara, Yoshio Fujii, Noriko Katoh, Chikako Tanaka (Department of Pharmacology, Kobe University School of Medicine), Masayuki Tsuji, Masamitsu Ichihashi and Yutaka Mishima (Department of Dermatology, Kobe University School of Medicine  /p.51
8.  Microdetermination of ¹⁰B₁ paraboronophenylalanine / Hiroshi Fujiwara, Yoshio Fujii, Chikako Tanaka (Department of Pharmacology, Kobe University School of Medicine), Masamitsu Ichihashi and Yutaka Mishima (Department of Dermatology, Kobe University School of Medicine)  /p.55
9. RBE(Relative Biological Effectiveness) of Thermal Neutron Capture Therapy of Malignant Melanoma Compared with 9MeV Electron Beam Irradiation / Junichi Hiratsuka, Shuji Kimura (Department of Radiology, Kobe University School of Medicine), Hiroshi Karashima (Hyogo Medical Center for Adults) and Yutaka Mishima (Department of Dermatology, Kobe University School of Medicine)  /p.59
10. Clinical Dosimetry Using Human Phantom with Skeletal Bone for Thermal Neutron Capture Therapy / Hiroshi Karashima (Hyogo Medical Center for Adults), Junichi Hiratsuka (Department of Radiology, Kobe University School of Medicine) and Yutaka Mishima (Department of Dermatology, Kobe University School of Medicine)  /p.63
11. Imaging of Boron-IO Distribution in Tissue and It’s Quantitative Analysis Using a-track Autoradiography /  Masao Takagaki (Yamagata University School of Medicine), Yutaka Mishima, Masamitsu Ichihashi (Department of Dermatology, Kobe University School of Medicine), Hiroshi Fukuda and Taiju Matsuzawa (Department of Radiology and Nuclear Medicine, The Research Institute for Tuberculosis and Cancer, Tohoku University)  /p.69
12. Boronic, Diboronic and a-Amino.-boronic Acids Derived from l-Ethynyl-3, 4 dimethoxybenzene / Toshia Nakagawa and Yu taka Mishima (Department of Dermatology, Kobe University School of Medicine)  /p.73
13. Clinical Trial with Fast Neutrons for malignant Melanoma / Hiroshi Tsunemoto, Shinroku Morita and Tatsuo Ishikawa (National Institute of Radiological Sciences)  /p.83
14. Therapeutic Model in Nude Mice Carrying Transplanted Human Melanoma Line: Studies of Selective Thermal Neutron Capture Therapy / Hidekazu Tamauchi, Norikazu Tamaoki (Department of Pathology, Tokai University School of Medicine), Masato Ueda and Yutaka Mishima (Department of Dermatology, Kobe University School of Medicine)  /p.91
15. Shared Antigenic Determinant Expressed on Various Mammalian Melanoma Cells / Masaru Taniguchi (Department of Immunology, Chiba University School of Medicine) and Seiji Wakabayashi (Department of Dermatology, Chiba University School of Medicine)  /p.95
16. Sandwich Radioimmunoassay Using Single Monoclonal Antibody That Detects Minute Amounts of Melanoma Antigens from Various Mammalian Species / Seiji Wakabayashi, Shoji Okamoto (Department of Dermatology, Chiba University School of Medicine) and Masaru Taniguchi (Department of Immunology, Chiba University School of Medicine)  /p.105
17. Thermal Neutron Flux Distribution in Phan tom / Toshikazu Shibata, Takashi Sato and Koichi Ono (Research Reactor Institute, Kyoto University  /p.111
18. Progress of Physics and Technology for Neutron Capture Therapy-Development of Measurement of ppm-order ¹⁰B Concentrationin vivo and Estimation of Effect of (γ, n) Reaction of Heavy Water on Neutron Field / Keiji Kanda, Tooru Kobayashi and Kazuhiko Aoki (Research Reactor Institute, Kyoto University)  /p.117
19. Selective Irradiation Technique and Dose Estimation for Neutron Capture Therapy/ Tooru Kobayashi and Keiji Kanda (Research Reactor Institute, Kyoto University)  /p.133
20. Analytical Calculation of Boron-10 Dosage in Cell Nucleus for Neutron Capture Therapy /Tooru Kobayashi and Keiji Kanda (Research Reactor Institute, Kyoto University)  /p.145
21. Microanalysis System of ppm-order ¹⁰B Concentrations in Tissue for Neutron Capture Therapy by Prompt Gamma-ray Spectrometry / Tooru Kobayashi and Keiji Kanda (Research Reactor Institute, Kyoto University)  /p.163
22. Phantom Experiment and Analysis for in vivo Measurement of Boron-10 Concentrations in Melanoma for Boron Neutron Capture Therapy / Kazuhiko Aoki, Tooru Kobayashi, and Keiji Kanda (Research Reactor Institute, Kyoto University)  /p.171
23. Biological Approach to the Killing Effect of Thermal Neutron Capture Therapy on Cultured B-16 Melanoma Cells / Goro Eguchi, Ryuji Kodama, Kiyokazu Agata, Tsuneyoshi Kuroiwa (The National Institute for Basic Biology, Department of Developmental Biology), Mitsumasa Okamoto (Institute of Molecular Biology, Faculty of Science, Nagoya University), Masamitsu Ichihashi and Yutaka Mishima (Department of Dermatology, Kobe University school of Medicine)  /p.181
24. Regeneration of the Lens as a Phenomenon of Cellular Transdifferentiation : Regulability of the Differentiated State of the Vertebrate Pigment Epithelial Cell / Goro Eguchi (Institute of Molecular Biology, Faculty of Science, Nagoya University) and Yoshiaki ltoh (Laboratory for Biology, Aichi Medical University)  /p.191
25. RBEs of Thermal Neutron Capture Therapy: Estimation by Theoretical Calculation / Hiroshi Fukuda (Department of Radiology and Nuclear Medicine, The Research Institute for Tuberculosis and Cancer, Tohoku University), Tooru Kobayashi, Keiji Kanda (Research Reactor Institute, Kyoto University) and Taiju Matsuzawa (Department of Radiology and Nuclear Medicine, The Research Institute for Tuberculosis and Cancer, Tohoku University)  /p.197
26. Estimation of Fluence of Thermal Neutron Capture Therapy Using Cultured Melanoma Cell / Hiroshi Fukuda (Department of Radiology and Nuclear Medicine, The Research Institute for Tuberculosis and Cancer, Tohoku University), Tooru Kobayashi (Research Reactor Institute, Kyoto University), Masamitsu Ichihashi (Department of Dermatology, Kobe University School of Medicine) and Taiju Matsuzawa (Department of Radiology and Nuclear Medicine, The Research Institute for Tuberculosis and Cancer, Tohoku University)  /p.203
27. Cellular Damage and Repair in Thermal Neutron Cap-ture Therapy of Malignant Melanoma/ Hiroshi Utsumi(Radiation Biology Center, Kyoto University), Masamitsu Ichihashi and Yu taka Mishima (Department of Derma to logy, Kobe University School of Medicine)  /p.209
28. Enhancement of Thermal Neutron-Induced Killing Effect on HeLa Cells by Means of Three 10-Boron-Compounds / Yowri Jeno (Department of Experimental Radiology, Faculty of Medicine, Kyoto University)  /p.219
29. ¹¹B, ¹³C-NMR Study of the Complex Formation of Phenylboronate with Catechol and L-Dopa / Kazuo Yoshino, Satoshi Oguchi, Yuji Shimakata, Yoshihiro Mori (Department of Chemistry, Shinshu University), Makoto Okamoto, Masahiro Kotaka (Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology) and Hidetake Kakihana (Department of Chemistry, Sophia University)  /p.221
30. Study of Melanoma Seeking Agent, ¹⁰B₁-p-Boronophenylalanine・HCIby Chemical Determination of Trace Boron in Biological Materials / Kazuo Yoshino (Department of Chemistry, Shinshu University), Makoto Okamoto (Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology), Hide take Kakihana (Department of Chemistry, Sophia University), Yoshihiro Mori (Department of Chemistry, Shinshu University), Yutaka Mishima, Masamitsu lchihashi, Masayuki Tsuji and Takafumi Nakanishi (Department of Dermatology, Kobe University School of Medicine)）  /p.233
31. Spectrophotometric Determination of Trace Boron in Biological Materials after Alkali Fusion Decomposition /Kazuo Yoshino, Makoto Okamoto, and Hidetake Kakihana (Research Laboratory for Nuclear Reactors, Tokyo Institute of Technology) Takafumi Nakanishi, Masamitsu Ichihashi, and Yutaka Mishima (Department of Dermatology, Kobe University School of Medicine)  /p.247
32. Acute Toxicity of 1¹⁰B₁-Paraboronophenylalanine・HCIin Mice / Koh taro Taniyama, Hiroshi Fujiwara and Chikako Tanaka (Department of Pharmacology, Kobe University School of Medicine)  /p.251